Occurrences and phenotypes of RIPK3-positive gastric cells in Helicobacter pylori infected gastritis and atrophic lesions

Author's accepted version (postprint).This is an Accepted Manuscript of an article published by Elsevier in Digestive and Liver Disease on 02/05/2022.Available online: https://www.dldjournalonline.com/article/S1590-8658(22)00258-4/fulltextacceptedVersio

The effects of notch filtering on electrically evoked myoelectric signals and associated motor unit index estimates

<p>Abstract</p> <p>Background</p> <p>Notch filtering is the most commonly used technique for suppression of power line and harmonic interference that often contaminate surface electromyogram (EMG) signals. Notch filters are routinely included in EMG recording instrumentation, and are used very often during clinical recording sessions. The objective of this study was to quantitatively assess the effects of notch filtering on electrically evoked myoelectric signals and on the related motor unit index measurements.</p> <p>Methods</p> <p>The study was primarily based on an experimental comparison of M wave recordings and index estimates of motor unit number and size, with the notch filter function of the EMG machine (Sierra Wave EMG system, Cadwell Lab Inc, Kennewick, WA, USA) turned on and off, respectively. The comparison was implemented in the first dorsal interosseous (FDI) muscle from the dominant hand of 15 neurologically intact subjects and bilaterally in 15 hemiparetic stroke subjects.</p> <p>Results</p> <p>On average, for intact subjects, the maximum M wave amplitude and the motor unit number index (MUNIX) estimate were reduced by approximately 22% and 18%, respectively, with application of the built-in notch filter function in the EMG machine. This trend held true when examining the paretic and contralateral muscles of the stroke subjects. With the notch filter on vs. off, across stroke subjects, we observed a significant decrease in both maximum M wave amplitude and MUNIX values in the paretic muscles, as compared with the contralateral muscles. However, similar reduction ratios were obtained for both maximum M wave amplitude and MUNIX estimate. Across muscles of both intact and stroke subjects, it was observed that notch filtering does not have significant effects on motor unit size index (MUSIX) estimate. No significant difference was found in MUSIX values between the paretic and contralateral muscles of the stroke subjects.</p> <p>Conclusions</p> <p>The notch filter function built in the EMG machine may significantly reduce the M wave amplitude and the MUNIX measurement. However, the notch filtering does not jeopardize the evaluation of the reduction ratio in maximum M wave amplitude and MUNIX estimate of the paretic muscles of stroke subjects when compared with the contralateral muscles.</p

Genome-wide identification and functional analysis of lincRNAs acting as miRNA targets or decoys in maize

LincRNA information derived from three articles. (XLS 20 kb

The expression of RIPK3 is associated with cell turnover of gastric mucosa in the mouse and human stomach

publishedVersio

Dynamic stromal cellular reaction throughout human colorectal adenoma-carcinoma sequence : A role of TH17/IL-17A

publishedVersio

Evaluation of anti-TNF therapeutic response in patients with inflammatory bowel disease : Current and novel biomarkers

publishedVersio

The presentation and regulation of the IL-8 network in the epithelial cancer stem-like cell niche in patients with colorectal cancer

Background: Accumulative evidence suggests that the biological behavior of cancer stem-like cells (CSCs) is regulated by their surrounding niche, in which cytokines function as one of the main mediators for the interaction between CSCs and their microenvironment in the colorectal cancer (CRC). Methods: We characterized the presentation of CSCs and the interleukin (IL)− 8 network in the adenoma/CRC epithelium using quantitative real-time PCR (q-PCR), immunohistochemistry (IHC) and double immunofluorescence. In addition, the capacity of IL-1β to stimulate epithelial IL-8 production in colon cancer Caco-2 cells was examined in vitro and the IL-8 product was measured by enzyme-linked immunosorbent assay (ELISA). Results: IHC observation showed increased expression of both CSCs and IL-8 in the adenoma and CRC epithelium, and q-PCR results revealed that increased expression of IL-1β transcript was strongly correlated with increased IL-8 transcript levels in both adenoma and CRC tissues. Double immunofluorescence images demonstrated the coexpression of the IL-8 receptors IL-8RA and IL-8RB with LGR5 labeled CSCs in CRC tissue sections. Consistently, in vitro experiments showed that coculture of Caco-2 cells with IL-1β at concentrations of 1, 5, 10 and 20 ng/ml resulted in a dose-dependent release of IL-8, which could be specifically inhibited by cotreatment with the IL-1β receptor antagonist. Conclusions: These results demonstrate activation of the IL-8 network in the niche of CSCs from the precancerous adenoma stage to the CRC stage, which is potentially stimulated by IL-1β in CRC cells

Temporal and spatial changes of cells positive for stem-like markers in different compartments and stages of human colorectal adenoma-carcinoma sequence

publishedVersio